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First off, I hit the streets of Eijsden, right? You know, that cute little town near the Maas river. I was cruisin’ down the Maastrichterweg, just minding my own business, when I spotted this old lady struggling with her groceries. I mean, c’mon, it’s like 2023, can’t we help each other out? So, I pulled over, hopped out, and helped her. She was super grateful, but then she started telling me her life story. I swear, I aged ten years listening to her ramble about her cat, Momo.
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Dissolved in THE RNA solution (Invitrogen) with an additional 20 mM DTT and heated to 60°C for 10′ to deactivate residual RNases. Isolated RNA was quantified using a Nanodrop spectrophotometer (ThermoFisher Scientific) and 250 ng/reaction was used for cDNA synthesis using oligo-DT primers. Quantitative qPCR was performed with a Lightcycler 480 (Roche Applied Science) with the small nuclear ribonucleoprotein D3 polypeptide (SNRPD3) as reference gene. Quantification was performed using the LinRegPCR method as described previously (van Scheppingen et al., 2016).Eijsden Erotic Massage
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