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But then, just as I’m getting into it, I hear a commotion. Some guy’s trying to start a fight. Ugh, typical. I rush over, and it turns out it’s just a misunderstanding. They were arguing over who had the best dance moves. I mean, really? So, I had to break it up, but I couldn’t help but chuckle.
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Isolated RNA was quantified using a Nanodrop spectrophotometer (ThermoFisher Scientific) and 250 ng/reaction was used for cDNA synthesis using oligo-DT primers. Quantitative qPCR was performed with a Lightcycler 480 (Roche Applied Science) with the small nuclear ribonucleoprotein D3 polypeptide (SNRPD3) as reference gene. Quantification was performed using the LinRegPCR method as described previously (van Scheppingen et al., 2016), outliers were removed using a ROUT algorithm with a Q = 0.5% and visualized in Graphad (8.4.3.Eijsden Erotic Massage
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