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After that, I decided to stroll down to Tamamura Park. It’s this chill spot with trees and a pond. I thought, “Hey, let’s relax.” But nah, some kids were playin’ soccer, and one of ‘em kicked the ball right at me. I dodged it like a ninja, but then I tripped over my own feet. So embarrassing! I’m layin’ there, and the kids are laughin’. I just laughed it off, but inside, I was like, “Why me?!”
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500 ng of pFucci-G1 Orange Expression vector was transfected into 293 A cells using Lipofectamine 3000 following the manufacturer’s protocol. Transfected cells were seeded on 35 mm glass bottom dish (Greiner Bio-One) 24 h after transfection using phenol red free high-glucose DMEM (Thermo Fisher Scientific) containing 10% FBS and penicillin/streptomycin.Tamamura Brothel
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