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GFP negative cells were sorted four times and the cells were cloned by limiting-dilution. Clone cells were treated with 10 ng/ml of TNF-α (R&D systems) or a combination of 1 μg/ml 5-Aza-dC (SIGMA-ALDRICH) and 1 μM TSA (SIGMA-ALDRICH) and screened for the potential to be reactivated after stimulation.Ebina Erotic Massage
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