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There’s a dive bar on Rue des Sables that’s raw and real. Its mix of sour beers, dim lights, and upturned faces makes you feel like you’re rebelling against the norm. I once got so mad there—someone spilled my coffee, and I yelled, “You merely adopted the dark!” before laughing it off. Life’s that mixed bag of anger and joy, ain’t it?
FcγR-mediated SARS-CoV-2 infection of monocytes activates inflammation
The codon-optimized gene for the expression of the RBD of SARS-CoV-2 (GenBank ID = MN975262.1; residues 319-529) was cloned into a pVRC vector containing a C-terminal HRV C3 protease cleavage site followed by 8x His and SBP tags. An additional C-terminal Cys residue was inserted using QuickChange Mutagenesis following the manufacturer’s protocol and mutagenesis was confirmed by next-generation sequencing (Azenta) to afford RBD-Cys.Boucau Prostitute
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